NAD+ 500mg — Clinical Reconstitution & Subcutaneous Administration Guide

For Healthcare Provider Use

Product Overview

500 mg Nicotinamide Adenine Dinucleotide (NAD+), lyophilized sterile powder in a 10 mL glass vial with flip-off cap. ≥99% purity (HPLC verified). Storage: 2–8°C. COA provided per batch.

Key roles: Cellular energy metabolism, mitochondrial function, redox balance, sirtuin activation.

Reconstitution Instructions

Reconstitute with Bacteriostatic Water for Injection (BWFI). Maintain aseptic technique. Do not use non-sterile water.

Option 1: 10 mL BWFI → 50 mg/mL

Volume Drawn	NAD+ Dose
1 mL	50 mg
2 mL	100 mg
5 mL	250 mg
10 mL	500 mg

Clinical note: 100 mg dose requires 2 mL injection.

Option 2: 5 mL BWFI → 100 mg/mL (preferred for SubQ comfort)

Volume Drawn	NAD+ Dose
0.5 mL	50 mg
1 mL	100 mg
2.5 mL	250 mg
5 mL	500 mg

Clinical note: 100 mg dose requires 1 mL injection.

Suggested Clinical Dosing

Common protocol: 100 mg subcutaneous injection twice weekly. At 50 mg/mL draw 2 mL; at 100 mg/mL draw 1 mL. Weekly total: 200 mg. Higher dosing may be divided across two injection sites.

Administration Steps

Recommended volume per site: 1–2 mL. Ideal concentration for comfort: 100 mg/mL. Rotate injection sites (abdomen, flank, posterior upper arm). Avoid exceeding 2 mL per single site.

Clean injection site with alcohol swab; allow to dry.
Draw dose with sterile syringe using aseptic technique.
Pinch skin at abdomen or thigh to create subcutaneous pocket.
Insert needle at 45–90° angle; inject slowly over 20–30 seconds per mL.
Withdraw needle; apply gentle pressure with gauze.
Dispose of syringe and needle in sharps container.

What to Expect

Injection site sensations

Mild burning or stinging (due to mildly acidic pH). Typically resolves within 5–15 minutes.

Temporary local reactions

Mild redness, warmth, firmness, slight swelling — resolves within hours.

Early systemic sensations

Increased mental clarity, improved focus, subtle mood elevation, enhanced daytime energy. Effects may be subtle and cumulative over 2–4 weeks. Individual results may vary.

Pre-Administration Checklist

Verify patient identity
Confirm lot number matches COA
Check expiration/BUD
Verify solution clarity
Confirm injection site clean
Ensure consent and documentation current

Storage & Handling

2–8°C

Refrigerate after reconstitution

Protect from light

Store in original packaging

Clinic Protocol

Follow facility BUD policy

Important Clinical Notes

Inspect for particulates before administration
Solution should be clear and colorless
Use new syringe per injection
Rotate injection sites
Document lot number and expiration in patient record